The effect of erythropoietin on albumins levels during hypoxia reoxygenation injury in rats

  • Tsompos Constantinos Consultant A, Department of Obstetrics & Gynecology, Mesologi County Hospital, Etoloakarnania,
  • Constantinos Panoulis
  • Konstantinos Toutouzas
  • George Zografos
  • Apostolos Papalois
Keywords: hypoxia, reoxygenation, erythropoietin, albumins

Abstract

The aim of this experimental study was to examine the effect of erythropoietin on rat model and particularly in an hypoxia reoxygenation (HR) protocol. The effect of that molecule was studied biochemically using blood mean albumins levels.

Materials and methods: 40 rats of mean weight 247.7 g were used in the study. Albumins levels were measured at 60 min (groups A and C) and at 120 min (groups B and D) of reoxygenation. Erythropoietin was administered only in groups C and D.

Results were that Epo administration significantly decreased the albumins levels by 9.28%+3.20% (p=0.0054). Reperfusion time non-significantly increased the albumins levels by 3.09%+3.52%  (p= 0.3405). However, erythropoietin administration and reperfusion time together produced a significant combined effect in keeping decreased the albumins levels by 5.37%+2.73% (p= 0.0072).

Conclusions are that erythropoietin administration whether it interacted or not with reoxygenation time, has significant decreasing effects on albumins levels in a short-term context of 2 hours.

Downloads

Download data is not yet available.

References

C. Τsompos, C. Panoulis, K. Τοutouzas, G. Ζografos, A. Papalois. The Effect of Erythropoietin on Total Protein Levels during Ischemia Reperfusion Injury in Rats. Int J Neurorehabilitation 2015; 2(1): 146.

Liepinsh E, Makrecka M, Kuka J, et al: The heart is better protected against myocardial infarction in the fed state compared to the fasted state. Metabolism. 2013 Oct 17. pii: S0026-0495(13)00298-9.

Abubakar S, Sabir A, Ndakotsu M, et al: Low admission serum albumin as prognostic determinant of 30-day case fatality and adverse functional outcome following acute ischemic stroke. Pan Afr Med J. 2013;14:53.

Herisson F, Delaroche O, Auffray-Calvier E, et al: Ischemia-modified albumin and heart fatty acid-binding protein: could early ischemic cardiac biomarkers be used in acute stroke management? J Stroke Cerebrovasc Dis. 2010 Jul-Aug;19(4):279-82.

Consuegra-Sanchez L, Bouzas-Mosquera A, Sinha MK, et al: Ischemia-modified albumin predicts short-term outcome and 1-year mortality in patients attending the emergency department for acute ischemic chest pain. Heart Vessels. 2008 May;23(3):174-80.

Polk JD, Rael LT, Craun ML, et al: Clinical utility of the cobalt-albumin binding assay in the diagnosis of intestinal ischemia. J Trauma. 2008 Jan;64(1):42-5.

Dusek J, Tichý M, Stásek J, et al: Ischemia-modified albumin: new marker of myocardial ischemia?. Cas Lek Cesk. 2005;144(5):295-7; discussion 297.

Worster A, Devereaux PJ, Heels-Ansdell D, et al: Capability of ischemia-modified albumin to predict serious cardiac outcomes in the short term among patients with potential acute coronary syndrome. CMAJ. 2005 Jun 21;172(13):1685-90.

Rafael Sadaba J, Conroy JL, Burniston M, et al: Effect of radial artery harvesting on tissue perfusion and function of the hand. Cardiovasc Surg. 2001 Aug;9(4):378-82.

Steinbauer M, Guba M, Büchner M, et al: Impact of polynitroxylated albumin (PNA) and tempol on ischemia/reperfusion injury: intravital microscopic study in the dorsal skinfold chamber of the Syrian golden hamster. Shock. 2000 Aug;14(2):163-8.

Donaldson GC, Robinson D, Allaway SL. An analysis of arterial disease mortality and BUPA health screening data in men, in relation to outdoor temperature. Clin Sci (Lond). 1997 Mar;92(3):261-8.

Arend SM, Bax JJ, Hermans J, et al: The short-term effect of intravenous nitroglycerin on haematocrit; an additional benefit in patients with myocardial ischaemia? Eur Heart J. 1994 Jan;15(1):114-9.

Pollock DM, Opgenorth TJ. Beneficial effect of the atrial natriuretic factor analog A68828 in postischemic acute renal failure. J Pharmacol Exp Ther. 1990 Dec;255(3):1166-9.

Tilton RG, Cole PA, Zions JD, et al: Increased ischemia-reperfusion injury to the heart associated with short-term, diet-induced hypercholesterolemia in rabbits. Circ Res.

Apr;60(4):551-9.

Kojima E, Hoshi H, Watanabe Y, et al: Daily hemodialysis improves uremia-associated clinical parameters in the short term. Contrib Nephrol. 2012;177:169-77.

Capelli JP, Kushner H. Correlates affecting survival in chronic hemodialysis patients: the combined impact of albumin and high hemoglobin levels on improving outcomes, local and national results. Hemodial Int. 2008 Oct;12(4):450-62.

Costa E, Lima M, Alves JM, et al: Inflammation, T-cell phenotype, and inflammatory cytokines in chronic kidney disease patients under hemodialysis and its relationship to resistance to recombinant human erythropoietin therapy. J Clin Immunol. 2008 May;28(3):268-75.

Lin CL, Hsu PY, Yang HY, et al: `Low dose intravenous ascorbic acid for erythropoietin-hyporesponsive anemia in diabetic hemodialysis patients with iron overload. Ren Fail. 2003 May;25(3):445-53.

Aguilera A, Bajo MA, Díez JJ, et al: Effects of human recombinant erythropoietin on inflammatory status in peritoneal dialysis patients. Adv Perit Dial. 2002;18:200-5.

Ortega O, Rodriguez I, Gallar P, et al: Significance of high C-reactive protein levels in pre-dialysis patients. Nephrol Dial Transplant. 2002 Jun;17(6):1105-9.

Rudduck C, Garson OM. The proportion of erythroid mitoses in normal human bone marrow in short-term culture systems. Pathology. 1989 Jul;21(3):185-8.

Krystal G, Eaves CJ, Eaves AC. CM Affi-Gel Blue chromatography of human urine: a simple one-step procedure for obtaining erythropoietin suitable for in vitro erythropoietic progenitor assays. Br J Haematol. 1984 Nov;58(3):533-46.

Published
2015-11-25
Section
Original Articles

Most read articles by the same author(s)

Obs.: This plugin requires at least one statistics/report plugin to be enabled. If your statistics plugins provide more than one metric then please also select a main metric on the admin's site settings page and/or on the journal manager's settings pages.