Zero order and area under curve spectrophotometric methods for determination of Paracetamol in pharmaceutical formulation
Abstract
Objective: - A simple, accurate, precise and specific zero order and area under curve spectrophotometric methods has been developed for determination of Paracetamol in its tablet dosage form by using methanol as a solvent.
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 Methods: - (1) Derivative Spectrophotometric Methods: - The amplitudes in the zero order derivative of the resultant spectra at 248 nm was selected to find out Paracetamol in its tablet dosage form by using methanol as a solvent.
(2) Area under curve (Area calculation): -The proposed area under curve method involves measurement of area at selected wavelength ranges. Two wavelength ranges were selected 244-251 nm for estimation of Paracetamol.
Result & Discussion: -The linearity was found to be 5-25 μg/ml for Paracetamol. The mean % recoveries were found to be 100.8% andzero order derivative and area under curve method of Paracetamol.For Repeatability, Intraday pr 100.66% of ecision, Interday precision, % RSD were found to be 0.7325, 0.0183 and 0.9768, 0.8742 for zero order and 0.8429, 0.8263 and 0.8570, 1.8017 for area under curve method respectively. Limit of Detection and Quantitation were found to be 0.4238μg/ml and 1.6987μg/ml for zero order and 0.8968μg/ml and 2.4278μg/ml for area under curve method respectively. Assay results of market formulation were found to be 100.25% for zero order and 100.88% area under curve method respectively. The proposed method has been validated as per ICH guidelines and successfully applied to the estimation of Paracetamol in its Tablet dosage form.
Conclusion: - The developed methods can be concluded as accurate, sensitive and precise and can be easily applied to the pharmaceutical formulation.
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References
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