Alternative pre-treatment of raw molasses by metal complexing agents for citric acid productivity by Aspergillus niger

  • Shazia Ashraf Govt. Apwa College for Women Jail Road, Lahore, Pakistan
  • Sikander Ali Institute of Industrial Biotechnology, GC University Lahore
  • Ikram-ul- Haq Institute of Industrial Biotechnology, GC University Lahore

Abstract

The present study deals with the pre-treatment of sugar cane molasses for the enhanced production of citric acid by Aspergillus niger NG-4. For this purpose, the acids and metal complexing agents were added in the molasses medium, alternatively prior to heating at 90°C for 1 h. Among them, the maximum amount of citric acid (53.2 g/l) was produced when the ratio between H2SO4+K4Fe(CN)6 was maintained at 1.0:250 for the pre-treatment of cane molasses which is approximately 3.1 fold higher than the control (17.0 g/l citric acid). The kinetic parameters such as growth yield coefficients (Yp/s, Yp/x, Yx/s in g/g), volumetric rates (Qp, Qs, Qx in g/l/h) and specific substrate rates (qp, qs in g/g cells/h) of the research work were also undertaken. The value of Qp (0.134 g/l/h) is highly encouraging (p≤0.05).

Downloads

Download data is not yet available.
Published
2015-06-30
Issue
Vol 1 No 1 (2015): JABBR
Section
Original Articles

Copyright (c) 2015 Journal of Advances in Biological and Basic Research

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).