Quantitative Estimation of Biochemical and Inorganic Constituents Present in the GallStones
Abstract
Carcinoma of gallbladder has an unusual geographic distribution. Gallbladder cancer is the most common type of biliary tract cancer which is the fifth most common gastrointestinal tract cancer and sixth most common digestive tract malignancy. Thus, the study was carried out based on quantitative analysis of biochemical constituents (cholesterol, bilirubin, bile acids, fatty acids, phospholipids and soluble protein) and inorganic constituents (sodium, potassium, calcium, magnesium, inorganic phosphate, oxalate and chloride) in all the three types of gallstones and also compare the present findings with other researchers findings. The results indicated that out of 179 gallstones, 41 were of cholesterol type, 77 mixed and 61 of pigment type. Cholesterol content was found to be the major component in all the three types of stones. Also, cholesterol stones had higher content of total cholesterol, fatty acids, phospholipids and inorganic phosphates as compared to mixed stones and pigment stones. While in case of pigment stones bilirubin, bile acids, soluble protein, sodium, potassium, calcium were found to be higher as compared to cholesterol and mixed stones. The overall findings with respect to biochemical constituents resulted that the cholesterol saturation is mostly responsible for crystallization sequences in human gallbladder.
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References
G.R. Verma; A.K. Pandey; S.M. Bose and R. Prasad (2002) Study of serum calcium and trace elements in chronic cholelithiasis. ANZ Journal of Surgery, Vol. 72, No. 8, pp. 596 - 599.
C.S. Pundir; R. Chaudhary; K. Rani; P. Chandran; M. Kumari and P. Garg (2001) Chemical analysis of biliary calculi in Haryana. Indian Journal of Surgery, Vol. 63, pp. 370-373.
Nagase Masao; Tanimura Hiroshi; Setoyama Motoichi and Hikasa Yorinori (1978) Present features of gallstones in Japan: A collective review of 2,144 cases. The American Journal of Surgery. Vol. 135, No. 6), pp. 788–790.
Indian Council of Medical Research, ICMR (1996) Annual report of population based cancer registries of the national cancer registry programme, New Delhi. ICMR Publication. Vol. 18.
P. Chandran; N.K. Kuchhal; P. Garg and C.S. Pundir (2007) An extended chemical analysis of gallstones. Indian Journal of Clinical Biochemistry, Vol. 22, No. 2, pp. 145-150.
C.J. Tsai; M.F. Leitzmann; F.B. Hu; W.C. Willett and E.L. Giovannucci (2004) A prospective cohort study of nut consumption and the risk of gallstone disease in men. American Journal of Epidemiology, Vol. 160, pp. 961-968.
A.M. Jaraari; J. Peela and N.P. Trushakant (2010) Quantitative analysis of gallstones in Libyan patients. Libyan Journal of Medicine, Vol. 5, pp. 4627-4633.
C.C. Allain; L.S. Poon; C.S. Chan; W. Richmond and P.C. Fu (1974) Enzymatic determination of total serum cholesterol. Clinical Chemistry, Vol. 20, pp. 470-475.
S.R. Gambino (1965) In “Standard methods of clinical chemistryâ€. Meiter S. Ed., Academic Press, Vol. 5, pp. 55.
R.J.D. Henry and J.W. Winkelman (1974) Clinical chemistry principles and techniques. Harper and Row, 2nd edition.
C.H. Fiske and Y. Subba Row (1925) The colorimetric determination of phosphorous. Journal of Biological Chemistry, Vol. 66, pp. 375-400.
D.W. Neill and R.A. Neely (1965) Titan yellow method for magnesium. Journal of Clinical Pathology, Vol. 9, pp. 162 cited in Hawk’s Physiological Chemistry. Ed. B.L. Oster, 4th Edition; The Blackiston Division, McGraw Hill Book Co. pp. 1139.
R.G. Schoenfeld (1964) Colorimetric method for chloride determination. Clinical Chemistry, Vol. 10, pp. 533.
American Public Health Association, APHA (2010) Standard methods for the examination of water and wastewater, Washington DC.
Stern Shapiro (1953) Practical clinical biochemistry by Harrold Varley. 4th edition, Heinemann Professional Publishing Ltd., London.
J.B. Carey (1958) The serum trihydroxy-dihydroxy bile acid ratio in liver and biliary tract disease. The Journal of Clinical Investigation, Vol. 17, pp. 1494-1502.
D. Kumar; P. K. Garg and P. K. Tandon (2001) Clinical and biochemical comparative study of different types of common bile duct stones. Indian Journal of Gastroenterology, Vol. 20, No. 5, pp. 187-190.
T. Lamont (1983) Mucin glycoprotein content of human pigment stone. Hepatology, Vol. 3, No. 3, pp. 372-382.
M. Fracchia; S. Pellegrino and P. Secreto (2001) Biliary lipid composition in cholesterol microlithiasis. Gut, Vol. 48, No. 5, pp. 702-706.
U. Gustafsson; S. Sahlin and C. Einarsson (2000) Biliary lipid composition in patients with cholesterol and pigment gallstone and gallstone free subjects. European Journal of Clinical Investigation, Vol. 30, No. 12, pp. 1099-1106.
R. D. Soloway; B. W. Trotman; W. C. Maddrey and F. Nakayama (1986) Pigment gallstone composition in patients with hemolysis or infection/stasis. Digestive Diseases and Sciences, Vol. 31, No. 5, pp. 454-460.
J. P. Binette and M. B. Binette (2000) The proteins and the formation of gallstones. Clinica Chimica Acta, Vol. 296, No. 1-2, pp. 59-69.
T. Maki (1966) Pathogenesis of calcium bilirubinate gallstones: Role of E.coli beta-glucoronidase and coagulation by inorganic ions- Poly-electrolytes and Agitation. Annals of Surgery, Vol. 164, No. 1, pp. 90-100.
T. Nakama; T. Furusawa, H. Itoh and T. Hisadome (1979) Correlation of cholesterol and bilirubin solubilization in bile salt solution. Gasteroenterologia Japonica, Vol. 14, No. 6, pp. 565-572.
R. K. Tandon (1990) Current development in the pathogenesis of gallstones. Tropical Gastroenterology, Vol. 11, No. 3, pp. 130-139.
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