Antidiabetic and antilipidemic effects of Andrographis paniculata ethanolic root extract in alloxan induced diabetic rats

  • Ukpanukpong, R. U Biochemistry Department, College of Natural Sciences, Joseph Ayo Babalola University P.M.B 5006 Ikeji-Arakeji Osun State
Keywords: Body Weight, Blood Glucose, Lipid Profile, Total Protein, Serum Albumin

Abstract

This investigation was aimed at finding the antidiabetic and antilipidemic potentials of Andrographis paniculata roots extract in Alloxan induced diabetic rats. Sixty female rats were used for the study and diabetes was induced into 50 rats by intraperitoneal injection of Alloxan while 10 were not induced. They were shared into six (6) study groups of 10 rats each and designated as; positive control group (NDC) placed on water and feeds, negative control group (DC) Alloxan induced but untreated, diabetic group treated with insulin and 3 diabetic groups (DAP1, DAP2, DAP3) administered ethanolic root extract at varying concentration of 200mg/kg, 400mg/kg and 800mg/kg B.W respectively for 7 days. Fasting blood glucose and body weight indices were monitored during the experimental period. Animals treated with insulin and Andrographis paniculata root extract shows significant decrease in their glucose concentration compared with the diabetic control (DC) at (p<0.05) at varying concentration. There was a significant decrease in the LDL level of the treated groups as against an increment in DC with relations to the NDC group. TAG was significantly decreased and HDL was significantly increased in the extract treated Group at 800mg/kg but significantly increased at 200mg/kg, 400mg/kg and insulin group. Serum albumin was also increased in like manner at (p<0.05). Total protein for the treated groups were increased significantly compared to the diabetic control group.(DC) which was significantly reduced compared to the non-diabetic control group (NDC) at (p<0.05) at varying concentration. The extract was found to be non-toxic as seen by the extract fed normal rats of A. paniculata root extract. In this present findings, A. paniculata roots extract possessed significant antihyperglycemic effect, as it lowers glucose concentration in the blood and lipid profile activity in alloxan induced diabetic rat.

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References

1. Tapsell, LC., Hemphill, I. and Cobiac, L. (2006). "Health benefits of herbs and spices: the past, the present, the future". Med. J. Aust. 185 (4 Suppl): S4–24.
2. Lai, PK. and Roy, J. (June 2004). "Antimicrobial and Chemo-preventive properties of herbs and spices". Curr. Med. Chem. 5 (11): 1451–60.
3. Fabricant, DS. and Farn, NR. (2001). Value of plants used in traditional medicine for drug discovery Environmental Health Perspective. National Institute of Environmental Health Science 109: 69-75.
4. Swain and Tony, ED. (1968). Plants in the Development of Modern Medicine. Harvard University Press.
5. Edgar, J., Da S., Elias, B. and Adnan, B. (2002). "Biotechnology and the developing world". Electronic Journal of Biotechnology 5 (1).
6. Ahmed, A.M. (2002). History of diabetes mellitus. Saudi Med J (23): 373-378.
7. Patlak, M. (2002). New weapons to combat an ancient disease: treating diabetes. FASEB J 16: 1853
8. Maitra, A. and Abbas, AK. (2005). Endocrine system. Robbins and Cotran Pathologic basis of disease (7th ed.). Saunders, Philadelphia. 1156-1226.
9. National Institute of Health (1985). Guide for the Care and Use of Laboratory Animals. Vol. 14: 544-89
10. Lowry, OH., Rosebrough, HJ., Farr, AL. and Randall, RJ.(1951). The Original Method. Journal Biol. Chem 193:265.
11. Plummer, D.T.(1979). An Introduction to Practical Biochemistry. 2nd. ed. New Delhi, India. Tata McGraw-Hill Ltd.
Published
2017-05-10
Issue
Vol 2 No 6 (2016): JABBR
Section
Original Articles

Copyright (c) 2016 Journal of Advances in Biological and Basic Research

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