Indian E-Journal of Pharmaceutical Sciences <p style="text-align: justify;">Indian E-Journal of Pharmaceutical Sciences (IEJPS) E-ISSN:2454-5244 is an&nbsp;open-access,&nbsp;peer-reviewed online&nbsp;monthly&nbsp;journal.&nbsp;The aim of&nbsp;journal publish high quality articles in field of&nbsp;Pharmaceutical Sciences.</p> ASD Publisher en-US Indian E-Journal of Pharmaceutical Sciences 2454-5244 <p>Authors who publish with this journal agree to the following terms:</p> <ol type="a"> <li>Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a <a href="" target="_new">Creative Commons Attribution License</a> that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.</li> <li>Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.</li> <li>Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See <a href="" target="_new">The Effect of Open Access</a>).</li> </ol> A comparative evaluation of oxidative status of erythrocytes in normal and sickle cell disease patients <p><strong>&nbsp;</strong>It is well established that G6PD deficient individuals are subjected to high oxidative stress and, hence, stiffening of the cell membrane due to oxidation of important membrane proteins. This effect is pronounced in RBCs where G6PD is the sole producer of NADPH, an essential cofactor in the antioxidant defense mechanism. A different approach is to evaluate oxidative stress as the analysis of antioxidant concentrations. GSH can be oxidized, mainly to glutathione disulfide (GSSG), or can form glutathionylated proteins (PSSG). The measurement of GSG, GSSG give fundamental information on the intracellular redox status. Analytical methods based on spectrophotometry, HPLC, capillary electrophoresis, nuclear magnetic resonance, and mass spectrometry have been reported for the determination of glutathione in biological samples. Our study has concluded that the HPLC method to measure the concentration of reduced (GSH) and oxidized (GSSG) glutathione in the normal (healthy) and Sickle Cell Disease patients. Erythrocyte glutathione depletion has been linked to hemolysis and oxidative stress. Our study revealed that the total antioxidant status in steady-state sickle cell disease (SCD) patients and compared it with some healthy individuals and related it to certain hematological parameters and their recent clinical history. 15 (males &amp; females) adult SCD patients and 15 age-matched controls were studied. And we hypothesized that altered glutathione and glutamine metabolism play a role in this process. Total glutathione (oxidized &amp; reduced) were assayed in erythrocytes of 15 SCD patients and 15 healthy volunteers. Erythrocyte glutathione levels were significantly lower in SCD patients than in healthy volunteers. The ratio of erythrocyte GSH: GSSG correlated inversely to the oxidized levels of the erythrocytes. &nbsp;</p> Lakshmi Prabha M. Nathiya R ##submission.copyrightStatement## 2017-09-19 2017-09-19 3 2 08 11