Indian E-Journal of Pharmaceutical Sciences <p style="text-align: justify;">Indian E-Journal of Pharmaceutical Sciences (IEJPS) E-ISSN:2454-5244 is an&nbsp;open-access,&nbsp;peer-reviewed online&nbsp;monthly&nbsp;journal.&nbsp;The aim of&nbsp;journal publish high quality articles in field of&nbsp;Pharmaceutical Sciences.</p> ASD Publisher en-US Indian E-Journal of Pharmaceutical Sciences 2454-5244 <p>Authors who publish with this journal agree to the following terms:</p> <ol type="a"> <li>Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a <a href="" target="_new">Creative Commons Attribution License</a> that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.</li> <li>Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.</li> <li>Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See <a href="" target="_new">The Effect of Open Access</a>).</li> </ol> Microbiological Evaluation of Combinations of Extracts of Euphorbia abyssinica and Coleus species for Antibacterial Activity <p>Antibacterial activity of 50% methanol extracts of <em>Coleus species </em>and<em> Euphorbia abyssinica</em> were investigated singly and in combination against <em>Staphylococcus aureus, Escherichia coli, Salmonella typhi, Pseudomonas aeruginosa, </em>using agar diffusion, macrobroth dilution, Checkerboard and Time kill assays. The inhibition zone diameters (IZDs) produced by the two plant extracts singly ranged from 17mm to 24mm, and those produced by the combination ranged from 17mm to 29mm. In the macrobroth dilution assay, the MICs obtained for the individual plant extracts ranged between 1.95-25mg/ml. In the Time Kill assay of the individual plant extracts<em>, S. aureus</em> was killed by 3.91mg/ml concentration of <em>E. abyssinica </em>and<em> Coleus species</em> alone, (at 1MIC and 2MIC), respectively. The combination killed it at 3.695mg/ml, indicative of synergy.&nbsp; <em>S. typhi </em>was killed by 12.5mg/ml and 6.25mg/ml (1MIC) concentration of <em>E. abyssinica</em> stem-bark and <em>Coleus species</em>, respectively. It was, also, killed by 6.26-, 6.88-, 7.51-, 8.14- and 8.75-mg/ml respective concentrations of different combinations of the two plant extracts. This is, also, indicative of synergy. <em>P. aeruginosa </em>was inhibited by 2MIC (25 mg/ml and 9.38mg/ml) of <em>E. abyssinica</em> stem-bark and <em>Coleus species </em>extracts<em>,</em> respectively, but a combination of the two plant extracts killed at 5.32mg/ml concentration, again indicating synergy.&nbsp; Some of the combinations tested using the checkerboard method showed synergy while others were indifferent or antagonistic after 24hours of exposure. The control drug, Gentamycin, was effective against all the test bacteria. A comparison of two methods revealed that the checkerboard assay was significantly more sensitive than the Time Kill assay (P&lt;0.05).The results authenticate the ethno-medicinal uses of these plants.</p> Tarh, Jacqueline Ebob Iroegbu, Christian Ukwuoma ##submission.copyrightStatement## 2017-03-04 2017-03-04 3 1 01 07